Redefining Rockel

You know I never discuss female thermodynamics - Lou; Rescue Me

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The Film of the Week

The WORD of the Week

Matthew 3:7-10

But when he saw many of the Pharisees and Sadducees coming to where he was baptizing, he said to them: "You brood of vipers! Who warned you to flee from the coming wrath?

Produce fruit in keeping with repentance.

And do not think you can say to yourselves, 'We have Abraham as our father.' I tell you that out of these stones God can raise up children for Abraham.

The ax is already at the root of the trees, and every tree that does not produce good fruit will be cut down and thrown into the fire.

Stupid Fact of the Week

There was only one civilian casualty during the three-day Battle of Gettysburg

29 October, 2006

Meanwhile...

This is what My Sister is working on:

Role of the Pseudomonas aeruginosa dinB-Encoded DNA Polymerase IV in Mutagenesis.

Pseudomonas aeruginosa is a human opportunistic pathogen that chronically infects the lungs of cystic fibrosis patients and is the leading cause of morbidity and mortality of people afflicted with this disease. A striking correlation between mutagenesis and persistence of P. aeruginosa has been reported. In other well-studied organisms, error-prone replication by Y-family DNA polymerases contributes significantly to mutagenesis. Based on an analysis of the PAO1 genome sequence, P. aeruginosa contains a single Y-family DNA polymerase encoded by the dinB gene. As part of an effort to understand mechanisms of mutagenesis in P. aeruginosa we have cloned the P. aeruginosa dinB gene, and utilized a combination of genetic and biochemical approaches to characterize the activity and regulation of the P. aeruginosa DinB protein (Pa-DinB). Our results indicate that Pa-DinB is a distributive DNA polymerase that lacks intrinsic proofreading activity in vitro. Modest overexpression of Pa-DinB from a plasmid conferred a mutator phenotype in both E. coli and P. aeruginosa. Examination of this mutator phenotype indicated that Pa-DinB has a propensity to promote C-->A transversions, and -1 frameshift mutations within poly-dGMP and poly-dAMP runs. Characterization of lexA(+) and DeltalexA::aacC1 P. aeruginosa strains, together with in vitro DNA binding assays utilizing cell free extracts or purified P. aeruginosa LexA protein (Pa-LexA) indicated that transcription of the dinB gene is regulated as part of an SOS-like response. Deletion of the Pa-dinB gene sensitized P. aeruginosa to nitrofurazone and 4-nitroquinoline 1-oxide, consistent with a role for Pa-DinB in translesion DNA synthesis over N(2)-dG adducts. Finally, P. aeruginosa exhibited a UV-inducible mutator phenotype that was independent of Pa-dinB function, and instead required polA and polC, which encode DNA polymerases I and the second DNA polymerase III enzyme, respectively. Possible roles of the P. aeruginosa dinB, polA and polC gene products in mutagenesis are discussed.

posted by Rockel @ 5:20 AM

3 Comments:

At 29/10/06 10:34 AM, Chuck Wade said…

huh?
At 29/10/06 12:04 PM, andiewade said…

*eyes glaze over as a small drop of drool appears at the corner of her mouth*
At 31/10/06 5:20 AM, Doyle said…

People with too much spare time :)

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Lyrics of the Week

ON THE NICKEL
by Tom Waits

("I'd like to do a new song here. This is eh, it's about downtown Los Angeles on 5th Street. And eh all the winos affectionately refer to it as The Nickel. So this is kind of a hobo's lullaby.")

sticks and stones will break my bones,
but i always will be true, and when
your mama is dead and gone,
i'll sing this lullabye just for you,
and what becomes of all the little boys,
who never comb their hair,
well they're lined up all around the block,
on the nickel over there.

so you better bring a bucket,
there is a hole in the pail,
and if you don't get my letter,
then you'll know that i'm in jail,
and what becomes of all the little boys,
who never say their prayers,
well they're sleepin' like a baby,
on the nickel over there.

and if you chew tobacco, and wish upon a star,
well you'll find out where the scarecrows sit,
just like punchlines between the cars,
and i know a place where a royal flush,
can never beat a pair, and even thomas jefferson,
is on the nickel over there.

so ring around the rosie, you're sleepin' in the rain,
and you're always late for supper,
and man you let me down again,
i thought i heard a mockingbird, roosevelt knows where,
you can skip the light, with grady tuck,
on the nickel over there.

so what becomes of all the little boys,
who run away from home,
well the world just keeps gettin' bigger,
once you get out on your own,
so here's to all the little boys,
the sandman takes you where,
you'll be sleepin' with a pillowman,
on the nickel over there.

so let's climb up through that button hole,
and we'll fall right up the stairs,
and i'll show you where the short dogs grow,
on the nickel over there.